Fig. 4: Application of on-demand synthesized phosphormamidites in oligonucleotide synthesis.
Top: Single coupling of phosphoramidites prepared on-demand to a resin-bound T7 followed by elongation with another T7 segment and cleavage from the resin. Bottom: Synthesis of a 51-mer using solely the on-demand approach. HPLC chromatograms of crude material: by conventional solid-phase synthesis with stored phosphoramidites (green) or with the on-demand synthesized phosphoramidites (blue). 51-mer sequence: 5′CCG CTT TCT AGT TCG TCC TCC ATA ATT AAT TTC CTA GAG TCC TAC GTG CTC 3′. B (on-demand synthesized phosphoramidite), CPG (controlled pore glass).
