Fig. 1: Feeding, rather than light controls susceptibility to LPS.
From: Feeding-induced resistance to acute lethal sepsis is dependent on hepatic BMAL1 and FXR signalling
a Graphic of the experimental setup illustrating light and feeding cycles. b Respiratory exchange ratio (RER) in female TRF mice over time housed in metabolic cages. Black bars indicate dark phases. DF: Food available during light phase only, NF: Food available during dark phase only, AL: Ad Lib, food available continuously. Representative of 7 individual experiments. N = 4. Data are presented as mean values ± SEM. Full statistical analysis using CalR in Supplementary Table 1. RER values min: DF = 0.65 NF = 0.67 AL = 0.71, max: DF = 1.32 NF = 1.28 AL = 1.23. c, d Morbidity in the night-time (c) or day-time fed (d) C57BL/6J and Bmal1fl/fl mice following 20 mg/kg LPS i.p. administered at ZT0 or ZT12 on day 5 of TRF with the indicated schedule. Statistical analysis according to Mantel–Cox: NF 0 vs. 12: p = 0.0015, DF 0 vs. 12: p < 0.0001, ZT0 NF vs. DF: p = 0.0004, ZT12 NF vs. DF: p < 0.0001, DF-ZT0 vs. NF-ZT12: p = 0.8376, DF-ZT12 vs. NF-ZT0: p = 0.0143. Experimental numbers: DF-ZT0-LPS Nfemale = 20 Nmale = 16, NF-ZT0-LPS Nfemale = 21 Nmale = 12, DF-ZT12-LPS Nfemale = 20 Nmale = 14, NF-ZT12-LPS Nfemale = 21 Nmale = 14, DF-ZT0-PBS Nfemale = 11 Nmale = 4, NF-ZT0-PBS Nfemale = 11 Nmale = 5, DF-ZT12-PBS Nfemale = 10 Nmale = 5, NF-ZT12-PBS Nfemale = 8 Nmale = 6. e–j Serum cytokines 6 h (e–i) or 2 h (j) after LPS stimulation (20 mg/kg) of female mice on day 5 of TRF at ZT0 or ZT12 as indicated. Box indicates mean. No significance between LPS-treated samples within feeding (ZT0 vs. ZT12) or stimulation time (DF vs. NF) according to two-way ANOVA and Sidak’s multiple comparisons. Experimental numbers: e DF-ZT0-LPS N = 8, NF-ZT0-LPS N = 7, DF-ZT12-LPS N = 11, NF-ZT12-LPS N = 9, N(PBS) = 6. f–i DF-ZT0-LPS N = 10, NF-ZT0-LPS N = 11, N(PBS) = 6. h, i DF-ZT12-LPS N = 11, NF-ZT12-LPS N = 10, N(PBS) = 6. f DF-ZT12-LPS N = 10, NF-ZT12-LPS N = 9, N(PBS) = 6. g DF-ZT12-LPS N = 11, NF-ZT12-LPS N = 9, DF-ZT12-PBS N = 6, NF-ZT12-PBS N = 5. j N(LPS) = 5, N(PBS) = 3.
