Fig. 7: Feeding-related glucose metabolism and cytokine response are unaffected in FxrΔHep animals.
From: Feeding-induced resistance to acute lethal sepsis is dependent on hepatic BMAL1 and FXR signalling
a Serum glucose 6 h post 20 mg/kg LPS i.p. at ZT0 on day 5 of TRF in FxrΔHep and Fxrfl/fl control female mice. Statistical analysis using two-way ANOVA and Sidak’s multiple comparison (NF vs. DF) as indicated. Interaction F(1,24) = 0.4606, p = 0.5038, genotype F(1,24) = 0.09389, p = 0.7619, feeding F(1,24) = 18.28, p = 0.0003. **p = 0.0017. FxrΔHep mice: NF N = 5, DF N = 7. Fxrfl/fl: N = 8. b, c IL-6 (E) or IL-1β (F) 6 h post 20 mg/kg LPS i.p. at ZT0 on day 5 of TRF in FxrΔHep and Fxrfl/fl control female mice. Statistical analysis using two-way ANOVA and Sidak’s multiple comparison (NF vs. DF) as indicated. b Interaction F(1,24) = 0.3339, p = 0.5693, genotype F(1,24) = 0.9463, p = 0.3413, feeding F(1,24) = 11.4, p = 0.0027. FxrΔHep mice: NF N = 5, DF N = 6. Fxrfl/fl: NF N = 8, DF N = 7. *p = 0.0027. c Interaction F(1,24) = 0.6027, p = 0.4452, genotype F(1,24) = 4.13, p = 0.0533, feeding F(1,24) = 21.53, p = 0.0001. FxrΔHep mice: NF N = 6, DF N = 7. Fxrfl/fl: NF N = 7, DF N = 8. *p = 0.0286 **p = 0.0011. d Morbidity in night-time and day-time fed FxrΔHep (solid line) (NF vs. DF p = 0.021/ns) or Fxrfl/fl (dashed) mice (NF vs. DF p = 0.0026/p = 0.0099), following 35 mg/kg LPS i.p. on day 5 of TRF at ZT12. Statistical analysis according to Mantel–Cox/Gehan–Breslow–Wilcoxon. Experimental numbers: FxrΔHep mice: DF-LPS Nfemale = 11 Nmale = 3, NF-LPS Nfemale = 10 Nmale = 2, DF-PBS Nfemale = 3 Nmale = 2, NF-PBS Nfemale = 1 Nmale = 1. Fxrfl/fl: DF-LPS Nfemale = 9 Nmale = 3, NF-LPS Nfemale = 9 Nmale = 6, DF-PBS Nfemale = 1, NF-PBS Nfemale = 2 Nmale = 1. e, f IL-6 (e) or IL-1β (f) 6 h post 35 mg/kg LPS i.p. at ZT12 on day 5 of TRF in FxrΔHep and Fxrfl/fl female mice. No significance using two-way ANOVA. N = 4.
