Fig. 2: Derivation of a neutrotime signature across biological compartments.
a Low-dimensional embedding of neutrophils from bone marrow, blood and spleen in the same space using a diffusion map-based approach. b Diffusion map for each tissue. c Convergence between UMAP populations P1–P4 and diffusion map ordering, as well as neutrotime representation on the UMAP embedding. d Relative cell density along neutrotime in each organ. e RNA velocity vector field on the neutrotime embedding. f Validation of the neutrotime gene signature in developing HoxA9 cells (loge fold change at 96 h and 120 h of differentiation). g Distribution of neutrotime (derived from diffusion map), maturation score (derived from principal component analysis, see Supplementary Fig. 5a–c) and preNeu score in cells ordered along neutrotime. Lower panel indicates the rolling density of cells in each compartment along neutrotime. The scale indicated below also serves as legend for other figures in which cells are ordered along neutrotime on the x axis.
