Fig. 1: Tissue-specific and cell-specific gene expression.
From: bric à brac controls sex pheromone choice by male European corn borer moths
a Heat map showing tissue-specific, sex-specific, and strain-specific mean expression of Bap18, LIM, BgiB ago, and bab as determined by RT-qPCR. Red signifies higher expression; pp prepupal stage; d days. ANOVAs per candidate gene, and separately for each strain, yielded significant variation among all tissue by life-stage combinations, after a Benjamini–Hochberg multiple-test correction. Letters indicate significant differences in two-sided Tukey’s HSD post-hoc tests (P < 0.05). Z strain: Bap18 F = 44.83, df = 20, P = 4 × 10−16; BgiB F = 3.81, df = 20, P = 9.01 × 10−5; LIM F = 37.97, df = 20, P = 4 × 10−16; ago F = 52.11, df = 20, P = 4 × 10−16; bab F = 155.2, df = 20, P = 4 × 10−16; E strain: Bap18 F = 7.1, df = 18, P = 2.41 × 10−07; BgiB F = 7.13, df = 18, P = 1.26 × 10−07; LIM F = 181.8, df = 18, P = 4 × 10−16; ago F = 18.31, df = 18, P = 2.61 × 10−13; bab F = 26.29, df = 18, P = 7.35 × 10−16. b Double whole mount in situ hybridization of differentially labeled transcripts of bab in red (digoxigenin) and odorant receptors and co-receptor in green (biotin). Top images show cells co-expressing both bab and Orco in 4-day-old male pupal antenna. Bottom images show separate but neighboring cells expressing bab and OR7, OR4, or Orco (co-localization in the same sensillum) in 2-day-old adult male antenna. In merged images on the right, yellow indicates overlapping of the two signals. Successful demonstrations in pupal antennae were obtained 4 times for bab/Orco combinations. Successful demonstrations in adult antennae were obtained 22 times for bab/OR4 combinations, 8 times for bab/OR7 combinations and 7 times for bab/Orco combinations. The scale bar corresponds to 20 µm. Source data are provided as a Source Data file.
