Fig. 1: Acquisition and characterization of antibodies.
From: A SARS-CoV-2 antibody curbs viral nucleocapsid protein-induced complement hyperactivation
Serum antibody titers of six SARS-CoV-2 convalescent patients and a healthy person (ZH0081, non-COVID-19) to the SARS-CoV-2 S (a) and N (b) proteins measured by ELISA. All samples were performed in triplicates and mean were presented. Sorting of single plasma cells (c) with CD38 and CD27 double-positive B cells. d To minimize false positives, each of the S1 and N proteins labeled with Phycoerythrin-canin7 (PE-Cy7) and Brilliant Violet (BV421) was used to sort antigen-specific memory B cells by FACS. e Percentage of different isotypes, VH and VL gene families of 32 isolated N-reactive antibodies. f Number of mutations in nucleotides and amino acids in VH and VL (Vκ and Vλ) of 32 N-reactive antibodies and eight S-reactive antibodies (g). Length of the 32 N-reactive antibodies (h) and eight S-reactive antibodies (i) in H-CDR3. f–i Data are presented as dot and mean values.
