Fig. 5: Consummatory behavior decreases the activity of SuM neurons. | Nature Communications

Fig. 5: Consummatory behavior decreases the activity of SuM neurons.

From: Supramammillary neurons projecting to the septum regulate dopamine and motivation for environmental interaction in mice

Fig. 5

a Example histology showing tetrode bundle track (dotted yellow line) passing through SuM with the tip marked by lesion (dotted red circle) ending in dorsal MB. Green fluorescence is ChR2-eYFP expression. Scale bar = 200 µm. b Diagram showing operant sucrose-seeking procedure with cue discrimination. c Latencies to enter sucrose port (mean ± SEM; n = 6 mice) after leaver pressing and hearing either CS+ or CS− cues during training sessions. *p < 0.05, ***p < 0.001, ****p < 0.0001, Bonferroni test. d–f Top: Heatmaps of normalized firing rates of SuM neurons during lever presses that initiate CS+ or CS− events (d), nose poke entry (e), and nose poke withdrawing (f) that follow CS+ or CS− lever presses. Neurons are arranged in the same sequence from top to bottom across all heatmaps. Bottom: Traces indicate mean (±SEM) normalized firing rate of neurons that are categorized as increasing, decreasing, or non-responding to CS+ and CS− with the same time scale as the raster plot above. The percentage of neurons in each category is shown in the boxes below. Source data are provided as a Source Data file.

Back to article page