Fig. 7: SuM-to-septum GLU ICSS depends on DA transmission and induced BOLD signal distributions are similar between SuM-to-septum GLU and VTA-VStr DA pathway stimulation.

a Schematics showing sites of AAV injections and optic fibers. b Lever presses per session (mean ± SEM; SuM:Sept^vGlut2 n = 7 mice, VTA:VStr^DA n = 7 mice). In sessions 10–12, laser power was titrated for each individual mouse as to support ICSS rates ~200 active presses per session. c, d Effects of low and high doses of DAR1 (c) and DAR2 (d) antagonists on active and inactive lever-press rates and locomotor activity (mean ± SEM; SuM:Sept^vGlut2 n = 6 mice, VTA:VStr^DA n = 8 mice). See text for description of statistical differences. e Cartoon showing an anesthetized mouse with passive stimulation in an fMRI scanner. f Diagram of block design for experimenter-delivered stimulation-trains during discrete ON/OFF epochs. g BOLD response patterns (SuM:Sept^vGlut2 n = 7 mice, VTA:VStr^DA n = 6 mice). Coronal sections roughly correspond to Franklin and Paxinos Mouse Brain Atlas images 21, 26, 31, 35, and 41 (from left to right). Only data from diencephalic regions are shown, as the present fMRI method was not optimized for detecting lower brain structures such as the VTA. All BOLD responses shown are significant, p_corrected < 0.01, voxel-wise beta-weighted t-test against zero using 3dttest. Source data are provided as a Source Data file.