Fig. 8: The excitation of SuM-to-septum GLU neurons activates VTA DA neurons and vice versa. | Nature Communications

Fig. 8: The excitation of SuM-to-septum GLU neurons activates VTA DA neurons and vice versa.

From: Supramammillary neurons projecting to the septum regulate dopamine and motivation for environmental interaction in mice

Fig. 8

a Top: Schematics showing sites of AAV injections and optic fibers. Bottom: Mean traces of GCaMP transients evoked by the stimulation of SuM-to-MS GLU neurons for individual mice (n = 5). b Top: Schematics showing sites of AAV injections and optic fibers. Bottom: Mean traces of dLight transients evoked by the stimulation of MS GLU neurons for individual mice (n = 6). c Fluorophores induced by MS injection of AAV-FLEX-mGFP-2A-SYP-mRuby in Vglut2-Cre mice (n = 3). This vector fills the entire Cre (i.e., GLU)-containing cells with mGFP and their terminal boutons with mRuby. Scale bars, left two = 200 µm; scale bar, right = 50 µm. Top left: Example photomicrogram showing injection site. Bottom left: Example photomicrogram showing fluorophores expressed in the VTA. Right: Enlarged squared region showing terminal boutons in the VTA. Abbreviations: cc, corpus callosum; DBB, diagonal band of Broca; LS, lateral septum; MS, medial septum; PBP, parabrachial pigmented nucleus of the VTA; PN, paranigral nucleus of the VTA; RN, red nucleus; VStr, ventral striatum. d Top: Schematics showing sites of AAV injections and optic fibers. Bottom: Mean traces of dLight transients evoked by the stimulation of MS-to-VTA GLU neurons for individual mice (n = 6). Inset: Correlation coefficient between evoked dLight signals in the VStr and the ICSS levels in session 5.

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