Fig. 3: Eukaryotic T2SS components are localised in mitochondria. | Nature Communications

Fig. 3: Eukaryotic T2SS components are localised in mitochondria.

From: Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system

Fig. 3

a S. cerevisiae expressing G. okellyi and N. gruberi Gsp proteins as C-terminal GFP fusions stained with MitoTracker red CMX ROS to visualise mitochondria. b Expression of G. okellyi GspD and GspG2 with the C-terminal V5 Tag in T. brucei visualised by immunofluorescence; the cells are co-stained with MitoTracker red. c Cellular fractions of N. gruberi labelled by specific polyclonal antibodies raised against GspG1, GspEN2A and mitochondrial (alternative oxidase – AOX, TatC,) cytosolic (hemerythrin) marker proteins. d Immunofluorescence microscopy of N. gruberi labelled with specific polyclonal antibodies raised against GspG1 and GspEN2A, and co-stained with MitoTracker red. Scale bar (parts a–d), 10 µm. (for a–d, representative images of multiple, at least three, experiments are shown), e PCA analysis of 4198 proteins identified in the proteomic analysis of N. gruberi subcellular fractions differentially enriched in mitochondria. The cluster of mitochondrial proteins was defined on the basis of 376 mitochondrial markers. The boundaries of the cluster of co-purified peroxisomal proteins were defined by 26 peroxisomal markers.

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