Fig. 7: The protein interactions of mitochondrial GspG.
a The soluble part of the pseudopilin domain was purified to homogeneity on and ran through a gel filtration column, which demonstrated the stable monomeric state of the protein (representative image of three experiments is shown). b Thermophoresis of NT-647-labelled protein showed specific self-interaction of the domain (top); plotting of the change in thermophoresis yielded a Kd of 216 ± 15.1 nm, concentration of the labelled GspG was 10 nM (bottom), The error bars depict standard deviation; n = 3. c Positive interaction between the mitochondrial GspG protein and other mitochondrial T2SS subunits as determined by the BACTH assay, n = 6.
