Fig. 3: Molecular mechanisms underlying Nur77’s effects on the interactions of Smurf2 with Smad3 and ID1. | Nature Communications

Fig. 3: Molecular mechanisms underlying Nur77’s effects on the interactions of Smurf2 with Smad3 and ID1.

From: Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis

Fig. 3

a, g Re-co-immunoprecipitation assay to detect the trimeric interaction of Nur77, ID1, and Smurf2 in SW620 cells. The detailed procedure was presented in Supplementary Figure 2b and described in “Methods” section. LC light chain, HC heavy chain. b, c SW620 cells transfected with the indicated expression plasmids or siRNAs were treated with MG132 (20 μM) for 2 h. Protein interactions were examined by co-IP. si-ctr control siRNA, si-Nur77 Nur77 siRNA. d–f Protein interactions were examined in mouse colon tissues (d) and in SW620 cells (e, f). Data represent at least two independent experiments. Source data are provided as Source Data file.

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