Fig. 3: Antibacterial assessments.

a Images of the bacterial colonies formed by S. aureus and P. gingivalis contacted with Ti-OH and Ti-PAA-NCl. b Antibacterial rates against S. aureus and P. gingivalis contacted with Ti-OH and Ti-PAA-NCl. Significant differences between Ti-OH and Ti-PAA-NCl are marked by different letters (n = 3; P < 0.0001; Student’s t test). c SEM images of S. aureus and P. gingivalis on the surfaces of Ti-OH and Ti-PAA-NCl (scale bar = 2 μm). Fluorescent images exhibiting the live/dead distribution of d S. aureus and e P. gingivalis on the surfaces of Ti-OH and Ti-PAA-NCl (green for live cells, red for dead cells, scale bar = 50 μm). f Quantitative measurements of long-lasting and renewable antibacterial rates of Ti-PAA-NCl via CFU counting after samples were stored in PBS for different duration times (n = 3). The pink part gives the antibacterial rate of the rechlorinated product of Ti-PAA-NCl with 12 weeks of durations. g Quantitative measurement of the repeated and renewable antibacterial activities of Ti-PAA-NCl via CFU counting (n = 3). The pink part gives the antibacterial rate of the rechlorinated product of Ti-PAA-NCl with 27 cycles. All error bars = s.d.