Fig. 2: BAFF modifies white adipose lipid handling.

a White adipocytes stimulated in the presence or absence of recombinant BAFF (rBAFF; 500 ng/ml) and subjected to RNA-seq analysis. Ontology pathways and heat map of selected associated genes. b eWAT mRNA expression quantified by qPCR of indicated lipolysis genes in WT, RP105^−/− or BAFF-Tg mice after 20 weeks on a HFD. c–d White adipocytes treated with saline (NS), norepinephrine (1 µM), or rBAFF (500 ng/ml) for 24 hours. c Free glycerol. % increase relative to WT NS. d mRNA expression of indicated lipolysis genes. e CD-fed WT mice were treated i.p. with rBAFF (2 μg/mouse) every other day for 1 week. eWAT mRNA expression of indicated lipolysis genes. f Primary white adipocytes from WT mice were treated with saline (NS), norepinephrine (NE; 1 µM), or rBAFF (b 500 ng/ml) for 12 hours. Protein expression of hormone-sensitive lipase (HSL), phosphorlayted HSL (p-HSL), and α-tubulin loading control by western blot. g Free glycerol in supernatants of WT, BAFF-R^−/−, TACI^−/−, or BCMA^−/− adipocytes treated with saline (NS) or rBAFF (500 ng/ml). % increase relative to WT NS. a A single experiment, n = 2/condition. b Representative of three independent experiments, n = 3–6/condition. c–d Representative of three independent experiments, n = 3–6/condition. e Representative of two independent experiments, n = 3/condition. f Representative samples of a single experiment, n = 3. g Representative of two independent experiments, n = 3/condition. b–e, g For bar graphs, data represents mean±SEM. b–e, g Unpaired two-tailed Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source data file.