Fig. 7: Effect of BAFF and APRIL axis is conserved in human adipocytes.
From: A BAFF/APRIL axis regulates obesogenic diet-driven weight gain
a–b Human primary adipocytes isolated from persons undergoing bariatric surgery. Adipocytes were treated with saline (NS), rBAFF (500 ng/ml), or rAPRIL (500 ng/ml) for 24 hours as indicated. a Supernatant free glycerol quantified by colorimetric assay. b LIPE mRNA expression quantified by qPCR, normalized to NS. c PNPLA2 mRNA expression quantified by qPCR, normalized to NS. d BAFF and e APRIL systemic concentration in lean or obese persons quantified by multiplex. f BAFF and g APRIL systemic concentration correlation with BMI. h BAFF and i APRIL systemic concentration correlation with decrease in BMI 1-year after bariatric surgery. a–c Representative persons, n = 9–10/condition. d–g Representative patients, n = 4 lean and n = 30 obese. h–i Data combined, n = 22 persons undergoing bariatric surgery. a–c For bar and line graphs, data represents mean±SEM. d–e For box plots, the midline represents the mean, boxes represent the interquartile range and whiskers show the full range of values. a–e Unpaired two-tailed Student’s t test. a, c, d *p < 0.05, ****p < 0.0001. f–i Linear regression. Source data are provided as a Source data file.
