Fig. 6: NETs are present in human MIBC tumors and a high PMN to CD8 ratio is associated with worse overall survival.
From: Role of neutrophil extracellular traps in radiation resistance of invasive bladder cancer
a Schematic representation of retrospective study design. b Representative image of NETs staining in MIBC tumors, where NETs were observed in patients with persistent disease post-RT treatment compared to responders, two-sided Fischer’s exact test (n = 52% non responders, n = 6% responders). c Intratumoral PMN infiltration pre-RT and post-RT treatment in subset of patients whom were NET positive, unpaired two-tailed paired student t-test. d Pre-RT intratumoral PMN’s correlates with post-RT NETs, unpaired two-tailed Mann–Whitney test (p = 0.02) (NET + ve patients n = 9, NET−ve patients n = 23). e Representative image of the spatial distribution of NETs (H3Cit-purple, NE-blue) and CD8 (brown) observed in tumor of a non-responder, tumor region (T). f PMN to CD8 ratio pre-RT, data expressed as percentage of positive cells per tumor core, unpaired two-tailed Mann–Whitney test p = 0.0018 (n = 11 responders, n = 14 non-responders). g Overall survival of patients with low or high PMN to CD8 ratio, log rank test (Mantel-cox). Data represented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.
