Fig. 1: Biochemical assays for IiPLR1 and AtPrRs function.
From: Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
a IiPLR1 efficiently catalyzes the conversion of pinoresinol into lariciresinol and also catalyzes the conversion of lariciresinol into secoisolariciresinol. In contrast, AtPrR1/2 exhibit a substrate preference for pinoresinol, yet exhibit only weak activity (AtPrR1) or no activity (AtPrR2) for lariciresinol. b Phylogenetic tree of PLRs/PrRs from different species. c Conversion of pinoresinol into lariciresinol and then into secoisolariciresinol by recombinant IiPLR1, AtPrR1, and AtPrR2. The reaction products were analyzed by LC-MS. d Chromatograms for pinoresinol, lariciresinol, and secoisolariciresinol are denoted in black, blue, and red, respectively.
