Fig. 4: Percent conversion of pinoresinol to lariciresinol and subsequently to secoisolariciresinol by mutants of IiPLR1, AtPrR1 and AtPrR2.
From: Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
a Conversion of pinoresinol into lariciresinol and subsequently to secoisolariciresinol. b Conversion of lariciresinol into secoisolariciresinol. Data are mean ± s.d. (n = 3 independent experiments). Asterisk (*) indicates significant difference from the wild-type enzyme (P < 0.05) analyzed by one-way ANOVA with Tukey’s multiple comparisons test. Source data underlying Fig. 4a, b are provided as a Source Data file. c LC-MS determination of the products as catalyzed by IiPLR1, IiPLR1_V46A, AtPrR2 and AtPrR2_N98S.
