Fig. 1: SET domain of ASH1L is essential for efficient transformation by MLL fusion oncogenes. | Nature Communications

Fig. 1: SET domain of ASH1L is essential for efficient transformation by MLL fusion oncogenes.

From: Discovery of first-in-class inhibitors of ASH1L histone methyltransferase with anti-leukemic activity

Fig. 1: SET domain of ASH1L is essential for efficient transformation by MLL fusion oncogenes.

a Normalized colony counts from the round four of colony-forming assay performed in mouse bone marrow (BM) progenitor cells derived from the WT or ∆SET Ash1l mice and transduced with MLL-AF9, MLL-AF6, HOXA9/MEIS1 (HM-2), E2A-HLF or vector alone (MSCV). n = 2. b, c Representative pictures of colonies (b) or Wright-Giemsa stained cytospins (c) from the round four of colony assay performed in bone marrow cells derived from WT or ∆SET Ash1l mice and transduced with MLL-AF9 or HOXA9/MEIS1 (HM-2). The experiment was repeated twice with similar results. d Quantitative RT-PCR performed in BM cells derived from WT or ∆SET Ash1l mice and transduced with MLL-AF9 or MLL-AF6. Gene expression was normalized to Gapdh and gene expression changes in ∆SET Ash1l cells were referenced to the corresponding values in the WT Ash1l background. Data represent two independent experiments each performed in duplicates.

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