Fig. 3: High-frequency rheology on unperturbed and F-actin perturbed HeLa cells.
From: Rheology of rounded mammalian cells over continuous high-frequencies
a Response curves of an unperturbed HeLa cell measured in configuration MM, MS, and SS (Fig. 2a). MM shows the response curve of the actuated master and SS of the actuated slave microcantilever. MS shows the slave microcantilever responding to the force of the photothermally actuated microcantilever transmitted through the cell. The raw data of the corresponding functions \({\chi }_{{\rm{MM}}},{\chi }_{{\rm{MS}}},\) and \({\chi }_{{\rm{SS}}}\) is displayed in Supplementary Fig. 10. b Cell rheological responses measured for two single HeLa cells. The extracted storage \({E}_{{\rm{cort}}}^{{\prime} }\) (red) and loss \({E}_{{\rm{cort}}}^{{\prime} {\prime} }\) (black) moduli of HeLa cells can be fitted using a double power-law behavior (black and green lines, respectively). At frequencies >35 kHz, the microcantilever response is noisier leading to the scattering of the data. c Cell rheological responses measured for two HeLa cells perturbed with 500 nM LatA. The frequency at which \({E}_{{\rm{cort}}}^{{\prime} {\prime} }(f)\) = \({E}_{{\rm{cort}}}^{{\prime} }(f)\) descreases considerably, which describes the cells to increase viscousity. d Cell rheological responses measured for two HeLa cells perturbed with 50 µM CK666. The frequency at which \({E}_{{\rm{cort}}}^{{\prime} {\prime} }(f)\) = \({E}_{{\rm{cort}}}^{{\prime} }(f)\) increases, indicating a more elastic response of the cell.
