Fig. 1: Workflow of single-cell lipidomics with high structural specificity.
From: Single-cell lipidomics with high structural specificity by mass spectrometry
a Workflow of single-cell fixation and optional derivatization for subsequent MS analysis. b Workflow of single-cell MS analysis method that allowed multi-round sampling and data acquisition. DAESI: droplet-assisted electrospray ionization. c Precursor ion scan of m/z 184 spectrum of the extract of unfixed (left) and fixed (right) MDA-MB-231 cells. Internal standard (IS): PC 15:0-15:0 (m/z 706). d Remain of PCs after fixation. Error bar represents the standard deviation, n = 3. e Experimental setup of collective single cells photochemical derivatization. Source data are provided in a Source Data file.
