Fig. 5: Discrimination of gefitinib-sensitive and gefitinib-resistant human lung cancer cells.
From: Single-cell lipidomics with high structural specificity by mass spectrometry
a–c t-SNE plot of HCC827 and HCC827/GR6 single cells calculated by molecular lipids profile (a), sn-position isomer compositions (b), or C=C location isomer compositions (c). Two cluster areas were circled by k-means clustering results with a confidence probability of 0.95. d Cell number distribution in each cluster. e Comparison of the relative abundance of PC 32:1 with C16:1(Δ9) or C18:1(Δ11) in gefitinib-sensitive and gefitinib-resistant cells at cell population level. Error bar represents the standard deviation, n = 42 for gefitinib-sensitive cells and n = 39 for gefitinib-resistant cells. ***P < 0.001, determined by single-tailed t-test. f Relative abundance of C=C location isomers of PC 32:1 in HCC827 and HCC827/GR6 single cells. Source data are provided in a Source Data file.
