Fig. 1: MAO-A-deficient mice show reduced tumor growth associated with altered TAM polarization. | Nature Communications

Fig. 1: MAO-A-deficient mice show reduced tumor growth associated with altered TAM polarization.

From: Targeting monoamine oxidase A-regulated tumor-associated macrophage polarization for cancer immunotherapy

Fig. 1: MAO-A-deficient mice show reduced tumor growth associated with altered TAM polarization.

a QPCR analyses of Maoa mRNA expression in TAMs isolated from wild-type mice-bearing B16-OVA tumors. Monocytes (Mo) were isolated from peripheral blood of tumor-free and tumor-bearing mice (***p < 0.001). N = 4. b–j Studying B16-OVA tumor growth in Maoa WT and Maoa KO mice. b Experimental design. c Tumor growth (**p = 0.0038, ***p < 0.001). d Tumor volume at day 18 (**p = 0.0038). e–h FACS analyses of CD206 (e) (***p < 0.001), CD69 (f) (***p < 0.001), CD86 (g) (**p = 0.0064) and I-Ab (h) (*p = 0.0275) expression on TAMs at day 18. WT, n = 9; KO, n = 8. MFI mean fluorescence intensity. i, j QPCR analyses of immunosuppressive (Mrc1, **p = 0.0041; Chi3l3, ***p < 0.001 and Arg1, *p = 0.0339; i) and immunostimulatory (Il6, ***p < 0.001; Ccl2, ***p < 0.001 and Tnf, **p = 0.0036; j) signature genes mRNA expression in TAMs (n = 4). k–n scRNAseq analyses of tumor-infiltrating immune cells (TIIs) from Maoa WT and Maoa KO mice at day 14 post B16-OVA tumors challenge. k Uniform Manifold Approximation and Projection (UMAP) of single TIIs showing the formation of six cell clusters (TAM/Mono, T cell, NK cell, B cell, DC and pDC) from total CD45.2+ TIIs and five cell clusters (TAM_1, TAM_2, Mono_1, Mono_2 and Mono_3) from the TAM/Mono subpopulation. Each dot represents one single cell and is coloured according to cell types. Mono monocyte, NK natural killer cell, DC dendritic cell, pDC plasmacytoid dendritic cell. l UMAP of the TAM subpopulation, showing the formation of two clusters (TAM_1: Mrc1lowCd86high; and TAM_2: Mrc1highCd86low). Each dot represents one single cell and is coloured according to cell clusters. Ratios of TAM_1:TAM_2 are presented. m, n Violin plots of immunosuppressive (Mrc1 and Chi3l3; m) and immunostimulatory (Ccl2, Ccl7, Cd86, H2-Aa, and H2-Ab1; n) signature genes expression in single TAMs. Each dot represents an individual cell. Representative of 1 (k–n), 3 (a), and 5 (b–j) experiments. Analysed by one-way ANOVA (a) or by Student’s t test (c–j). p values of violin plots are determined by Wilcoxon rank-sum test (m, n). Statistics are all two-sided. Source data are provided as a Source Data file.

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