Fig. 1: Overview of the SPOT system.
From: A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis
a Overall workflow using capillaries and the SPOT device. Green and red dashed lines represent excitation spectrum of FAM and ROX, respectively, while emission spectrums are shown with green and red solid lines. Inset: Device and consumables needed to run the SPOT system, including a 3D printed portable detection device, a prefabricated sample pretreatment capillary 1, a prefabricated viral RNA detection capillary 2, and collection capillaries. A quarter coin is placed for scale (diameter: 24.26 mm); b Genome map showing primers, gDNAs, and SPOT mechanism. RT-LAMP primers are indicated by black rectangles. Enclosed “P” represents the phosphate group. PfAgo cleavage sites are shown in black triangles, enclosed “Q” represents quencher group and enclosed “F” represents fluorophore group.
