Table 1 Buffer compositions used for various chromatid reconstitution assays.

From: Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assembly

 

Supplementary Fig. 1

Figure 1a

Figure 1b

Figure 1c

Figure 2 afterward

X. laevis topo IIα [nM]

0–250

40

40

40

40

MgCl2 [mM]

2.0

0–5.0

5.0

5.0

5.0

KCl [mM]a

80

120

50–200

80

80 (150)

KH2PO4 [mM]

40

0

0

0

0

ATP [mM]

2.0

2.0

2.0

0–1.0

2.0

HEPES-KOH [mM], pH 7.7

20

20

20

20

20

  1. aThe KCl concentrations include their carryovers from storage buffers of protein preparations.
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