Fig. 8: Response to the chemical stimuli of “pure” and “mixed” oscillators.

a A schematic representation of the CSTR experimental set-up for studying the effect of chemical stimuli on chemical oscillators. b The expected reactions of furfuryl alcohol (33), glyoxal (34), and thiosemicarbazide (35) with the components of oscillators. c The influence of furfuryl alcohol (100 mM) on the “mixed” (left) and “pure” (right) oscillators. In the “mixed” oscillator, the mixture of 8 (25 mM) and acetylthiocholine (32) (25 mM) was used instead of pure 8, and the mixture of maleimide (5 mM) and K₃[Fe(CN)₆] (5 mM) was used instead of pure maleimide. Other experimental conditions—the concentrations of 17 (92 mM) and acrylamide (321 mM), PB pH 8, at 25 °C—were as in all previous experiments; f/V = 2.96 × 10⁻⁴ s⁻¹. We used the following experimental conditions in control experiments with the “pure” thiouronium salt-based oscillator: H₂O, 1 M PB pH 8, at 25 °C; [8] = 56 mM; [17] = 92 mM; [Acrylamide] = 321 mM; [Maleimide] = 6.2 mM; f/V = 2.15 × 10⁻⁴ s⁻¹. The numbers above plots show avarage periods and their standard deviations. d The influence of glyoxal (10 and 20 mM) on the “mixed” (left) and “pure” (right) oscillators. e The influence of thiosemicarbazide (10 mM) on the “mixed” (left) and “pure” (right) oscillators.