Fig. 6: Characterization of the Onset of locomotor phenotype.
Performance of SOD1G93A mice before and after Onset of locomotor phenotype is characterized by loss of speed (a) (two-tailed t test, P = 0.00008, t = 6.350, df = 10; N = 11 mice), reduction in peak acceleration (b) (two-tailed t test, P = 0.0118, t = 3.072, df = 10; N = 11 mice), decrease in stride length (c) (two-tailed t test, P = 0.0036, t = 3.779, df = 10; N = 11 mice) and step frequency (d) (two-tailed t test, P = 0.0004, t = 5.273, df = 10; N = 11 mice). Moreover, SOD1G93A mice showed increased dragging events when trying to cope with the speed of the belt, drag counts in (e) (two-tailed t test, P =0.0196, t = 2.776, df = 10; N = 11 mice) and drag duration in (f) (two-tailed t test, P =0.0068, t = 3.395, df = 10; N = 11 independent mice). Dotted lines show averages for wild-type (wt) mice in all parameters included in the analysis. g Quantifications of left-right alternation showed as circular plots. Perfect alternation corresponds to a phase of 180 degrees. Data from individual animals are plotted for each condition (orange-empty SOD1 pre-symptomatic, orange-full SOD1 onset, black wild-type). The mean vectors for each condition are represented in the respective colors. There is no difference in the mean phase for the different conditions (two-tailed Watson–Williams test, SOD1G93A pre-symptomatic vs SOD1G93A onset P = 0.1582; wt vs SOD1G93A pre-symptomatic P = 0.1004; wt vs SOD1G93A onset P = 0.3261; N = 11 mice, n = 15 steps per mouse). h Grip strength shows progressive but late decline of low force performance in the SOD1G93A starting from P90 (one-way ANOVA and Dunnett’s post hoc, F (4, 27) = 45.89, P90 P = 0.00009, P120 P = 0.00009, P140 P = 0.00009; P45 N = 4, N = 7 mice for all other timepoints). In all graphs, data are presented as mean values ± SEM. Source data are provided as a Source Data file.
