Fig. 9: Dampening of spinal V1 interneuron activity does not have an effect in SOD1G93A mice after Onset of locomotor phenotype. | Nature Communications

Fig. 9: Dampening of spinal V1 interneuron activity does not have an effect in SOD1G93A mice after Onset of locomotor phenotype.

From: Locomotor deficits in a mouse model of ALS are paralleled by loss of V1-interneuron connections onto fast motor neurons

Fig. 9

a Mouse crossing utilized to assess specific spinal V1 interneuron silencing in a SOD1G93A mouse model. SOD1G93A;En1cre mice were crossed with HoxB8FlipO;RC::FPDi. SOD1G93A mice that did not carry the intersectional expression were used as controls. After CNO administration quadruple transgenics (magenta bars) did not show changes in speed (b) (one-way ANOVA and Sidak’s post hoc, F(3, 18) = 0.02417, P = 0.9977; control N = 7 independent mice, quadruple transgenics N = 4 independent mice) nor in peak acceleration (c) (one-way ANOVA and Sidak’s post hoc, F(3, 18) = 0.08636, P = 0.9982; control N = 7 mice, quadruple transgenics N = 4 mice). Also stride length (d) (one-way ANOVA and Sidak’s post hoc, F(3, 18) = 1.211, P = 0.5915; control N = 7 mice, quadruple transgenics N = 4 mice) and step frequency (e) remained unchanged (one-way ANOVA and Sidak’s post hoc, F(3, 18) = 0.02689, P = 0.9602; control N = 7 mice, quadruple transgenics N = 4 mice). SOD1G93A;En1cre;HoxB8FlipO;RC::Di mice did not show more dragging events (f–g) after CNO administration (drag counts one-way ANOVA and Sidak’s post hoc, F(3, 18) = 1.914, P = 0.4302; drag duration one-way ANOVA and Sidak’s post hoc, F(3, 18) = 1.839, P = 0.5068; control N = 7 mice, quadruple transgenics N = 4 mice). h CNO administration did not have any effects on grip strength in SOD1G93A;En1cre;HoxB8FlipO;RC::Di mice (one-way ANOVA and Sidak’s post hoc, F(3, 18) = 0.1216, P = 0.9380; control N = 7 mice, quadruple transgenics N = 4 mice). SOD1G93A not carrying dual intersectional expression did not show any changes after CNO administration (orange bars). Dotted lines show averages for wild-type (wt) animals in all parameters included in the analysis. i Left-right alternation remained unaltered after CNO administration in both SOD1G93A;En1cre;HoxB8FlipO;RC::Di mice and SOD1G93A controls (two-tailed Watson–Williams test, P = 0.3682; n = 15 independent steps per mouse). In all graphs, data are presented as mean values ± SEM. Source data are provided as a Source Data file.

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