Table 1 ITC derived apparent dissociation constants for each step of the TAPBPR-mediated peptide exchange cycle.

TAX8 K_D1,app

37.4 nM

n/d

13.9 nM

n/d

TAX9 K_D1,app

30.6 nM

n/d

11.1 nM

n/d

KLL15 K_D1,app

38.8 nM

n/d

14.2 nM

n/d

TAX8 K_D2,app

326 ± 14 nM

0.43 ± 0.02

316 ± 19 nM

0.44 ± 0.03

TAX9 K_D2,app

40.2 ± 6.5 nM

0.42 ± 0.01

42.6 ± 8.3 nM

0.46 ± 0.01

KLL15 K_D2,app

383 ± 22 nM

0.45 ± 0.06

334 ± 20 nM

0.41 ± 0.01

TAX8 K_D3

2.6 ± 0.5 µM

1.00 ± 0.03

2.4 ± 0.6 µM

1.03 ± 0.01

TAX9 K_D3

1.9 ± 0.7 µM

1.02 ± 0.01

1.8 ± 0.3 µM

1.06 ± 0.02

KLL15 K_D3

2.2 ± 0.5 µM

1.05 ± 0.04

2.1 ± 0.3 µM

1.03 ± 0.01

TAX8 K_D4

22.7 ± 8.2 µM

1.01 ± 0.02

54.4 ± 9.4 µM

1.05 ± 0.02

TAX9 K_D4

2.5 ± 0.8 µM

1.04 ± 0.01

7.3 ± 0.7 µM

1.02 ± 0.02

KLL15 K_D4

21.6 ± 7.0 µM

1.10 ± 0.05

49.5 ± 8.6 µM

1.03 ± 0.04