Fig. 1: Preparation of gold nanoparticle/multi-functional 3D DNA self-assembled multilayer core/shell nanostructure (siRNA/Ap-CS) and its application for targeted siRNA delivery and controlled release within tumor cells. | Nature Communications

Fig. 1: Preparation of gold nanoparticle/multi-functional 3D DNA self-assembled multilayer core/shell nanostructure (siRNA/Ap-CS) and its application for targeted siRNA delivery and controlled release within tumor cells.

From: Programmably tiling rigidified DNA brick on gold nanoparticle as multi-functional shell for cancer-targeted delivery of siRNAs

Fig. 1

Step I, functionalization of AuNP with thiol-modified anchoring DNA complementary to miRNA (ADC), forming ADC-AuNP; Step II, binding of elongated siRNA to AuNP-confined ADC, producing siRNA/ADC-AuNP; Step III, programmed positioning of aptamer (Ap)-integrated Y-shaped backbone-rigidified triangular DNA bricks (Ap-YTDB) onto siRNA/ADC-AuNP, leading to the formation of expected biocompatible core/shell nanostructure, siRNA/Ap-CS; Step IV, aptamer-mediated specific cell recognition and endocytosis; Step V, intracellular controlled release of encapsulated siRNAs by the strand displacement initiated by endogenous miRNAs.

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