Fig. 5: Colocalization assay of siRNA (red fluorescence) and AuNPs (black dot) within target cancer cells. | Nature Communications

Fig. 5: Colocalization assay of siRNA (red fluorescence) and AuNPs (black dot) within target cancer cells.

From: Programmably tiling rigidified DNA brick on gold nanoparticle as multi-functional shell for cancer-targeted delivery of siRNAs

Fig. 5

MCF-7 cells were separately incubated with releasable siPlk1/Ap-CS (a), unreleasable siPlk1 (un-siPlk1)/Ap-CS (b) and releasable siPlk1/CS without aptamer (c) for 4 h. d is the same as a but L02 cells were instead used. HM in the right half part is the high-resolution image of the area in yellow dotted box indicated in the section of Merge, while HBF is the high-resolution image boxed in bright field (BF). AuNPs in HM and HBF are highlighted by yellow dotted circles (a–d). The cell imaging experiments in a–d were conducted three times independently with similar results. e Flow cytometry analysis of MCF-7 cells treated with various formulations for 4 h. f The quantitative fluorescence intensity of each sample in e. The error bar represents the standard deviation (SD). The measured data are expressed as the means ± SD (n = 3).

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