Fig. 1: Liver- specific PPDPF deficiency leads to the development of fatty liver disease.
From: PPDPF alleviates hepatic steatosis through inhibition of mTOR signaling
a The body weight of PPDPF-LKO (n = 6) and WT (n = 6) mice at 8 months. Mean ± SEM, ***p = 0.0005 by two-tailed unpaired Student’s t test. b The ratio of liver-to-body weight (LW/BW) of PPDPF-LKO (n = 6) and WT (n = 6) mice at 8 months. Mean ± SEM, *p = 0.003 by two-tailed unpaired Student’s t test. c Liver images of WT and LKO mice at 8 months of age. d Images of H&E and Oil Red O staining of liver tissues from the WT and LKO mice at 8 months. Scale bars, 100 μm. e, f The triglyceride (TG) and nonestesterified fatty acid (NEFA) in the livers of WT and LKO mice at 8 months of age (n = 6 mice for each group). Mean ± SEM, e **p = 0.002, f **p = 0.001 by two tailed unpaired Student’s t test. g The relative mRNA levels of genes involved in lipid metabolism, including lipogenesis genes, oxidation-related genes, lipid uptake, and lipid secretion in WT and LKO mice at 8 months of age (n = 6 for each group). The mRNA expression levels of the genes are normalized to that of 18 s. Mean ± SEM, SREBP1: *p = 0.025, FASN: *p = 0.035, ACLY: *p = 0.045, ME: *p = 0.012, PPARG: *p = 0.011 by two-tailed unpaired Student’s t test. h Representative images of immunohistochemical staining of PPDPF in liver sections from controls and NAFLD patients. Scale bars,100 μm. All experiments were repeated three times independently.
