Fig. 1: miR-802 regulates glucose absorption in the small intestine.
From: miR-802 regulates Paneth cell function and enterocyte differentiation in the mouse small intestine
a Relative expression levels of miR-802 in indicated tissues (jejunum n = 4,4; liver n = 7,6; kidney n = 7,6; pancreatic islet n = 5,1; cortex n = 4,4). Data are representative of three independent experiments. b Relative miR-802 expression in female mice, measured by qRT-PCR, in indicated intestine sections (n = 6). Data are representative of two independent experiments c Oral glucose tolerance test (OGTT) of mir-802KO and control littermates (WT) (n = 8,9 per genotype). d, e OGTT (d) and IPGTT (e) in Vil-Cre mir-802fl/fl and mir-802fl/fl controls (n = 8,6 respectively for (d), n = 6 for (e)). f, g Serum GLP1 and insulin measurements of Vil-Cre mir-802fl/fl and control mice at the indicated time points after the oral glucose challenge (for (f) n = 7 for each genotype, for (g): wt n = 6 and KO n = 9). h Relative mRNA levels of Gip and Glp1 in small intestines of Vil-Cre mir-802fl/fl and control mice (n = 10 per group). i GLP1 protein measurements of jejunum from Vil-Cre mir-802fl/fl and control mice (n = 7 per group, one experiment). j–l D-14C-Glucose levels in proximal jejunum (j), distal jejunum (k), and serum (l) following an oral 14C-glucose gavage (n = 8 for all groups, one experiment). m Heatmap representation of mRNA expression by RNA seq of glucose transporters in isolated jejunal enterocytes of Vil-Cre mir-802fl/fl and control mice shown as Log2FC (n = 3). n Relative mRNA expression, measured by qRT-PCR, of indicated glucose transporters in jejunal enterocytes of Vil-Cre mir-802fl/fl and indicated control mice (n = 9 per genotype). Data are representative of two independent experiments. o Immunoblot of GLUT2 in isolated enterocytes of the proximal jejunum of Vil-Cre mir-802fl/fl and control mice. γ-TUBULIN was used as a loading control. Quantification of densitometric analysis of signals is shown on the right. Each lane represents a lysate from a different mouse (n = 4 per genotype, one experiment). Data are plotted as mean ± SD. Significance was evaluated by two-tailed t test (j–l, o), by multiple two-tailed t-tests with Holm−Sidak method for multiple comparisons (a, h, i), one-way ANOVA with Tukey’s multiple comparison post-test (n), or Dunnet post test (b), and two-way ANOVA for repeated measures with Sidak’s multiple comparisons test (c–g).
