Fig. 2: UV-irradiation experiments (λexc = 280 nm, 80 µW cm−2) of damaged DNA trinucleotides containing a T=T dimer and A or D.
From: 2,6-diaminopurine promotes repair of DNA lesions under prebiotic conditions
a Analytical HPLC analysis of irradiated DT=T, T=TD, AT=T, and T=TA performed after different irradiation times. The peaks at shorter retention times correspond to damaged trinucleotides, while the peaks at longer retentions correspond to the repaired photoproducts. b Differential absorption UV spectra demonstrating changes of the spectrum of the irradiated material at different irradiation times. Both trinucleotides containing D exhibit significant increase of absorption at 264 nm, which corresponds to the absorption maximum of repaired T chromophore. Only minor increase of absorption at 264 nm can be observed for the trinucleotides containing A.
