Fig. 1: SssI-targeted methylation at the FWA promoter cause silencing.
From: Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase
A Cartoon depicting the RNA-directed DNA methylation pathway. B Flowering time of untransformed controls, and T1 ZF-SssI lines in the fwa background or mutants that have been introgressed into the fwa background. The X-axis represents the number of leaves. Each dot represents one individual plant. Dashed lines indicate the cutoff we chose to define ‘early flowering’ versus ‘late flowering’ (*p-value < 0.01 compared to fwa, Welch two-sample t-test). C Flowering time of untransformed control lines and four representative ZF-SssI T2 lines in the fwa background or mutants that have been introgressed into the fwa background (*p-value < 0.01 compared to relative controls, Welch two-sample t-test). D CG, CHG, and CHH methylation levels over the FWA promoter measured by bisulfite (BS)-PCR-seq in untransformed controls and ZF-SssI in the fwa background or mutants that have been introgressed into the fwa background. The barplot represents data from one representative T2 plant for each genotype tested. Every single bar represents one cytosine. Black triangles and orange shaded rectangle regions indicate the designed ZF binding sites. The relative position of the three regions analyzed in the FWA gene are indicated as blue squares. E Flowering time of untransformed Col-0, fwa, and two representative T3 ZF-SssI lines with (+) or without (‒) the transgene. (* p-value < 0.01 compared to fwa, Welch two-sample t-test). Source data underlying Figs. 1B, 1C, and 1E are provided as a Source Data file.
