Fig. 1: Bidirectional FtsZ treadmilling rings yield directional screw-like lipid membrane deformations.
From: FtsZ induces membrane deformations via torsional stress upon GTP hydrolysis
a FtsZ-YFP-mts ring structures externally decorating GUVs (scale bar = 10 µm). b After GUV deflation, inwards conical deformations emerged from FtsZ rings (GUVs: N > 20) (scale bar = 5 µm). c Inspired by deformations in (b), we designed a PDMS microstructure with inwards conical geometry covered with a supported lipid bilayer (SLB). The imaging plane was chosen to have a cross-section of 1–2-µm diameter (scale bar = 5 µm). d Inside cones, FtsZ-YFP-mts self-assembled into dynamic vortices (Supplementary Movie S1) (scale bar = 5 µm). e A representative kymograph (N > 3) showed negative and positive slopes indicating the presence of clockwise and anticlockwise directions.
