Fig. 1: High-throughput screen for activators of Yap signaling.

a A schematic diagram demonstrates the strategy for the identification of compounds that promote the nuclear transit of Yap, thus reversing the exclusion characteristic of contact inhibition. b The number of MCF 10A cells rises almost linearly with their confluency (n = 3 for each condition, mean ± SEM). c Nuclear localization of Yap falls systematically with increasing confluency in MCF 10A cultures (n = 3 for each condition, mean ± SEM). d Immunolabeling of Yap (red) demonstrates nuclear localization of the protein in a positive-control (subconfluent) culture but not in a negative-control (confluent) culture. Treatment of a confluent culture with a representative hit compound promotes extensive movement of Yap into nuclei. The experiment was conducted four times with the same results. See also Supplementary Fig. 1.