Fig. 3: Comparison of E1–E2 ectodomains structure and sequence from MAYV and CHIKV.

A Structural alignment of E1 and E2 domains from MAYV (blue and purple) and CHIKV (grey). The shorter MAYV loop in the E1 III subdomain is highlighted in the right inset. Details of the loop connecting the E2 A and B subdomains, including the missing asparagine in MAYV, are shown in the left inset. B E1–E2 dimer coloured by conservation in relation to CHIKV and aligned with CHIKV structure in complex with Fab fragments from CHIKV-neutralizing antibodies CHK-9, CHK-152, m242 and m1040. C The interaction sites for the four antibodies are highlighted in red in the MAYV structure. The conservation map on the antibody interaction surface is highlighted, with non-conserved residues in MAYV labelled in cyan.