Fig. 6: MB2mP6 inhibits CLP-induced lung vascular leakage induced by CLP sepsis.
From: Targeting Gα13-integrin interaction ameliorates systemic inflammation
a Representative images of septic mouse lungs 24 h after CLP. The mice were treated with scrambled peptide (Scra) or MB2mP6 immediately after CLP onset. At 23.5 h after CLP, the mice were i.v. injected with Evans blue albumin (EBA; 1%, 25 mg kg−1 body weight). After 30 min, the mouse lungs were harvested, and the Evans blue was extracted by incubating with formamide at 65 oC for 18 h. The EBA contents in the lung tissues were calculated using a standard EBA curve detected at 620 nm absorbance with 740 nm reference (sham, n = 4; control, n = 5; MB2mP6, n = 4, independent animals). b, Representative images of septic mouse lungs 24 h after CLP treated with Scrambled peptide or MB2mP6 6 h after CLP onset. The Evans blue contents in septic mouse lung 24 h after CLP were analyzed as in (a) (sham, n = 4; control, n = 6; MB2mP6, n = 6, independent animals). All data are shown as mean ± SEM. Data were analyzed by one-way ANOVA with Tukey’s multiple comparisons test.
