Fig. 3: Compartmentalization and localization of DNA inside the evaporating droplet.

DNA localizes into dextran-rich compartments inside the evaporating sessile droplet. DNA is labeled with Cy-5 (red) and dextran-rich compartments are labeled with FITC-dextran (green). The ATPS solution is made up of 9 wt% PEG and 4 wt% dextran, with the addition of Cy-5 labeled DNA (10 µM) and FITC-dextran (\({M}_{w}=4000,\) 0.5 wt%). Images are obtained over analysis of seven independent trials with relative humidity ranging from 55 to 65%. The scale bar is 500 μm.