Fig. 4: In vitro transcription of functional RNA aptamers inside the evaporating sessile droplets. | Nature Communications

Fig. 4: In vitro transcription of functional RNA aptamers inside the evaporating sessile droplets.

From: Non-associative phase separation in an evaporating droplet as a model for prebiotic compartmentalization

Fig. 4: In vitro transcription of functional RNA aptamers inside the evaporating sessile droplets.

a The flow of genetic information in phase-separated compartments via in vitro transcription (IVT). The DNA templates are coded for Broccoli aptamer that can bind 5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) to form a fluorescent complex of Broccoli–DFHBI. b Optical image sequence shows fluorescence RNA aptamer transcription from Broccoli DNA template inside the phase-separated compartments. The dextran-rich compartments have increasing green fluorescence due to the formation of Broccoli-DFHBI complexes. Images are obtained over analysis of three independent trials with relative humidity ranging from 55 to 65%. The scale bar is 500 μm.

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