Fig. 5: Methylation analysis confirms the strong IFN signature in C1 and C3 and reveals an aberrant methylation status in C4.
From: A new molecular classification to drive precision treatment strategies in primary Sjögren’s syndrome
Whole blood methylation analysis was performed for 226 pSS patients (C1: 81, C2: 57, C3: 62, and C4: 26) and 175 healthy volunteers (HV) doing pairwise comparisons between each cluster and HV. a Venn diagram showing the overlap of differentially methylated CpG sites and genes between the 4 clusters with absolute ΔBeta > 0.075. b DMP distribution across the different genomic regions (gene body, 3’UTR, intergenic (IGR), 5’UTR, Exon 1, TSS 1500 and TSS 200; and according to the CpG density to CpG island, shelf, shore, and open sea. c Venn diagram showing the overlap of hypomethylated CpG and genes with absolute ΔBeta > 0.15 between the three IFN clusters. d Interaction network of these 5 genes common to the three clusters by STRING analysis with a confidence cut-off of 0.4 reveals a common IFN signature. e Interaction network of the 10 genes hypomethylated common to C1 and C3 by STRING analysis with a confidence cut-off of 0.4. f Reactome analysis22 of the functional pathways enriched for the 126 genes hypomethylated and over expressed in C4 (absolute ΔBeta > 0.15, FC ≥ 1.5).
