Fig. 6: Domain organization of the NRPSs CaeA2, ColA2, and ColA2PKS-CaeA2NRPS (Top) and HPLC analysis of COL-related products in S. roseosporus strains (below).

i, the wild-type COL-producing strain (positive control); ii, the ΔcolA2 mutant in which a chimeric gene colA2PKS-caeA2NRPS is expressed in trans; and iii, the ΔcolA2 mutant (negative control).