Fig. 1: Effect of IP6 on RSV in vitro and in cells. | Nature Communications

Fig. 1: Effect of IP6 on RSV in vitro and in cells.

From: Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer

Fig. 1

a RSV Gag and truncation proteins used for assembly and structure determination. Alignment of RSV and HIV-1 amino acid sequences. Known HIV-1 IP6-interacting residues shown in red and predicted RSV IP6-interacting residues indicated (red arrow). b TEM images of immature RSV Gag-∆MBD assembly without and with IP6 at low and high magnification. c TEM images of mature RSV CASPNC assembly without and with IP6 at low and high magnification. d RSV infectious virus particle production in wild type (HEK293T) and IPPK-KO cell lines. e (left) Western blot normalized band intensity, and (right) representative western blots of of cell lysate and GAPDH control (top) and virus fractions (bottom). Uncropped western blots are shown in Supplementary Fig. 1. Box plots: center lines show the medians, box limits indicate the 25th and 75th percentile; whiskers extend to minimum and maximum values; data points are plotted as circles. Source data are provided with this paper.

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