Fig. 2: Cryo-electron tomography of RSV CASPNC CLPs assembled in the presence of IP6. | Nature Communications

Fig. 2: Cryo-electron tomography of RSV CASPNC CLPs assembled in the presence of IP6.

From: Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer

Fig. 2

a Sum of ten computational slices through a gaussian-filtered tomogram containing CASPNC tubes and CASPNC polyhedrons. Scale bar is 100 nm. The slices are representative of the 49 tomogram dataset. b, c Isosurface representation of the subtomogram average of a C2-symmetric CA hexamer from the CASPNC tubes (b) and a C5-symmetric pentamer from CASPNC polyhedrons (c). In both cases, the CANTD and CACTD of one CA monomer are colored cyan and orange, respectively. The C2 and C5 symmetry of the hexamer and pentamer are annotated by a distorted schematic hexamer and a pentamer, respectively. The resolution of the individual structures is annotated. See corresponding FSC curves in Supplementary Fig. 3a. d Model of the IP6 binding site in the CA-NTD hexamer pore formed by amino acids K17 (green) and R21 (purple), as seen from the outside of the CLP (left) and rotated by 90° (right). The side chains are shown with their most likely rotamer position in agreement with the EM density.

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