Fig. 3: Building blocks of the mature CASPNC lattice.
a A composite model of a representative RSV CASPNC CLP, generated by placing respective unit pair averages (see groups in b) into their positions derived from subtomogram alignment. The models shown in a and in Supplementary Movie 3 are identical. b Final averages of unit pairs filtered to 8 Å. Groups are identical to their schematic depiction in Supplementary Fig. 4. Coloring of the groups corresponds with a. c Pairwise RMSD measurements between rigid-body fitted models derived from the maps of the different classes shown in b. In case of CANTD–CANTD, CANTD–CACTD, and CACTD dimer interfaces, one CA domain is fixed and RMSD is measured for the other CA domain across the respective interface. For the CACTD trimer interface one CA domain is fixed and RMSD is measured for the other two CACTD’s forming the trimer interface. d Superimposition of the models showing the flexibility of CA domains involved in the different interfaces. Color code: cyan to blue—CANTD; yellow to red—CACTD. Source data are provided with this paper.
