Table 1 Data collection and model refinement statistics.
From: Architecture of the Sema3A/PlexinA4/Neuropilin tripartite complex
Data collection and processing | |
|---|---|
Magnification | 46,730 |
Voltage (kV) | 300 |
Electron exposure (e−/Å2) | 50 |
Defocus range (µm) | 1.5–2.5 |
Pixel size (Å) | 1.08 |
Symmetry imposed | C2 |
Map resolution (Å) | 3.7 |
FSC threshold | 0.143 |
Model refinement | |
Initial model used (PDB code) | 5l5k, 4gz8 & 2qqk |
Model resolution (Å) | 4.2 |
FSC threshold | 0.5 |
Map sharpening B factor (Å2) | −30 |
Model composition | |
Non-hydrogen atoms | 30,898 |
Protein residues | 4228 |
Ligands | 4 |
B factors (Å2) | |
Protein | 260 |
Ligand | 216 |
R.m.s. deviations | |
Bond length (Å) | 0.004 |
Bond angle (°) | 0.7 |
Validation | |
Molprobity score | 2.06 |
Clashscore | 17.4 |
Poor rotamers (%) | 1 |
Ramachandran plot | |
Favored (%) | 95.6 |
Allowed (%) | 4.4 |
Outliers (%) | 0 |
