Fig. 3: The transcriptional diversity among SMTs.

a Illustration of the anatomical position of SMTs. a A total of 47 types of SMTs were used to compare transcriptional profiles between anatomical regions, classified into four general groups: two head SMTs (orange) (LIN: Lingualis, MAS: Masseter); 14 forelimb SMTs (green) (BB: Biceps brachii, DAP: Deltoideus acromial part, DSP: Deltoideus scapular part, EDC: Extensor digitorum communis, EDL(F): Extensor digitorum lateralis (forelimb), FDP(F): Flexor digitorum profundus (forelimb), FDS(F): Flexor digitorum superficialis (forelimb), ISP: Infraspinatus, SSP: Supraspinatus, SUB: Subscapularis, TB-LA: Triceps brachii caput lateralis, TB-LO: Triceps brachii caput longum, TB-M: Triceps brachii caput medialis, TM: Teres major); 11 trunk SMTs (red) (BRA: Brachiocephalicus, LD: Latissimus dorsi, LDM: Longissimus dorsi muscle, OEA: Obiliquus extensor abdominis, PP: Pectoralis profundus, PS: Pectoralis superficialis, RA: Rectus abdominis, RHO: Rhomboideus, SP: Splenius, TA: Transversus abdominis, TRC: Trapezius cervicis); and 20 hindlimb SMTs (blue) (ADD: Adductores, BF: Biceps femoris, EDL(H): Extensor digitorum lateralis (hindlimb), FDP(H): Flexor digitorum profundus (hindlimb), FDS(H): Flexor digitorum superficialis (hindlimb), GSA-L: Gastrocnemius-lateralis, GAS-M: Gastrocnemius-medialis, GM: Gluteus medius, GRA: Gracillis, PM: Psoas major, RF: Rectus femoris, SAR: Sartorius, SM: Semimembranosus, SOL: Soleus, ST: Semitendinosus, TFL: Tensor fasciae latae, TIC: Tibialis cranialis, VI: Vastus intermedius, VL: Vastus lateralis, VM: Vastus medialis). b–f Similarity of global PCG transcription (b) and transcriptional profiles between SMTs from different anatomical regions for HOX genes (c), homeobox family genes (d), secreted myokines (e), and neuromuscular junction (NMJ) assembly genes (f) based on Spearman’s correlation (upper panel) and multidimensional scaling distances determined by t-SNE (lower panel). Ellipses in the t-SNE plots indicate the four SMT groups, constructed at a probability of 0.95. Source data for b–f are provided as a Source Data file.