Fig. 4: MYST1 and SIRT2 control the level of PAX7 acetylation in satellite cells.
From: Acetylation of PAX7 controls muscle stem cell self-renewal and differentiation potential in mice
a Representative PAX7:acetylated-lysine PLA (red) performed on satellite cells on cultured myofibers following siRNA treatments against Myst1 or Sirt2, as indicated. Satellite cells are labeled with SYNDECAN-4 (SDC4, green) and nuclei are counterstained with DAPI (blue). Scale bar represents 10 μm. Images are representative of ≥75% of the cells examined (n = 3 mice). b, c Quantification of the PLA signals from (a), represented as the mean ± SEM. The PLA was quantified by counting the number of nuclear PLA puncta for each satellite cell. b (n = 69 siCtrl, n = 61 siMyst1 cells from 3 mice). c (n = 86 siCtrl, n = 77 siSirt2 cells from 3 mice) (Two-tailed paired t test: **p = 0.0082 in (b); **p = 0.0096 in (c)). d Representative PAX7:acetylated-lysine PLA (red) performed on satellite cells cultured on myofibers in presence of DMSO, AGK2 or MG149, as indicated. SDC4 marks the satellite cells (green) and nuclei are counterstained with DAPI (blue). Scale bar represents 10 μm. Images are representative of ≥75% of the cells examined (n = 3 mice). e Quantification of the PLA signals from (d), represented as the mean ± SEM. The PLA was quantified by counting the number of nuclear PLA puncta for each satellite cell (n = 107 DMSO, n = 107 MG149, n = 90 AGK2 cells from 3 mice).
