Fig. 8: PAX7 acetylation regulates target gene expression in satellite cells.

a Heatmap from normalized expression matrix of differentially expressed genes between Pax7^WT and Pax7^KR satellite cells. The number of PAX7 ChIP-seq peaks associated with each gene is indicated. Genes in bold with an asterisk are significantly different (Wald test followed by multiple testing correction using the Benjamini–Hochberg method to control the false discovery rate, with application of a cut-off of 0.1 (approximating to a p-value of 3.5 × 10⁻⁴) to corrected values). Heatmap coloring indicates row Z-score; color bars to the left indicate log2 fold change, and log2 mean expression. b Plot illustrating the distance from the closest PAX7 ChIP-seq peak and transcriptional start site (TSS) to the closest PAX7 ChIP-seq peak vs the negative log p-value for all PAX7 target genes. Differentially and significantly expressed genes between Pax7^WT and Pax7^KR satellite cells are highlighted in red (Wald test followed by multiple testing correction using the Benjamini–Hochberg method to control the false discovery rate, with application of a cut-off of 0.1 (approximating to a p-value of 3.5 × 10⁻⁴) to corrected values). c Motifs identified de novo in Pax7 peaks are similar to motifs described in Soleimani et al.¹⁰. The homeobox motif is enriched in PAX7 target genes (all genes), as well as in the subset that is differentially expressed in Pax7^KR satellite cells. In contrast, the paired motif is found only when analyzing peaks associated with all PAX7 target genes, but not when considering only the subset of genes differentially expressed in Pax7^KR satellite cells. Numbers indicate the percentage of peaks containing the different motifs, as well as the p value calculated by HOMER. d Gene ontology (GO) term enrichment analysis (biological process) for PAX7 target genes that are differentially expressed between Pax7^WT and Pax7^KR satellite cells.